Sunscreen activity of ethanol extract of mangrove fruit flour and its formulation in cream preparation

Sunscreen activity of ethanol extract of mangrove fruit flour and its formulation in cream preparation

Authors

  • Anintya Khaela Stifar Yayasan Pharmasi Semarang, Semarang, Indonesia
  • Mutmainah Mutmainah Stifar Yayasan Pharmasi Semarang, Semarang, Indonesia
  • Lia Kusmita Stifar Yayasan Pharmasi Semarang, Semarang, Indonesia

Keywords:

Sunscreen, Mangrove, Cream, Extract, Brayo

Abstract

Increased exposure to ultraviolet (UV) radiation has raised public awareness of the importance of using effective and safe sunscreens, especially those derived from natural ingredients. Mangrove ecosystems are a source of natural bioactive compounds that have the potential to act as photoprotective agents because they contain flavonoids, tannins, and phenolics that can absorb ultraviolet (UV) rays and act as antioxidants. This study aims to evaluate the potential sunscreen activity of Brayo (Avecenia marina) and Lindur (Bruguiera gymnorrhiza) extracts in raw and cooked forms, and to develop a cream formulation with the best extract. Extraction was carried out by remaceration using 70% ethanol solvent. The Sun Protection Factor (SPF) value of each extract was determined using UV spectrophotometry. The extract with the highest SPF results was then formulated into a cream preparation with concentrations F1 (1%), F2 (3%), and F3 (9%). Each formula was tested for physical characteristics including pH, viscosity, adhesion, and spreadability, and tested for sunscreen activity in vitro. The results showed that raw Brayo had the highest SPF value (24.47 ± 0.58) and was included in the ultra protection category. Organoleptic and homogeneity tests showed that all formulas met the requirements. For pH and adhesion tests, formulas 3 and 4 met the requirements, while formula 1 did not. Viscosity and spreadability tests for all formulas met the requirements. the SPF value increased with increasing extract concentration. Thus, raw Brayo extract has the potential as a natural active ingredient in sunscreen cream formulations with high effectiveness. These findings indicate that raw Brayo extract has the potential as a natural sunscreen agent that can be formulated stably in cream form for skin protection applications against UV radiation.

References

[1] Luger, T., Amagai, M., Dreno, B., Dagnelie, M.A., Liao, W., Kabashima, K., dkk. 2021. Atopic dermatitis: Role of the skin barrier, environment, microbiome, and therapeutic agents. Journal of Dermatological Science, 102: 142–157.

[2] Almuqati, R.R., Alamri, A.S., dan Almuqati, N.R. 2019. Knowledge, attitude, and practices toward sun exposure and use of sun protection among non-medical, female, university students in Saudi Arabia: A cross-sectional study. International Journal of Women’s Dermatology, 5: 105–109.

[3] Erliani, D., Sari, M., dan Islamiyati, R. 2023. Formulasi dan Penentuan Nilai SPF (Sun Protection Factor) Sediaan Krim Minyak Biji Kelor (Moringa oleifera L.). Cendekia Journal Of Pharmacy. 7(1): 67-78

[4] Slobodian, L., Buelow, C.A., Baker, S.C., Alvarez, S., Wood, K.C., Villarreal-Rosas, J., dkk. 2025. Quantifying the presence and potential of national legal frameworks for global mangrove protection. Cell Reports Sustainability,2(8) 100430.

[5] Nugroho, W., Ayuchecaria, N., dan Aryzki, S. 2022. Penentuan Nilai Sun Protecting Factor (SPF) Dan Uji Karakteristik Sifat Fisik-Kimia Sediaan Nanoherbal Bedak Dayak. Journal Pharmaceutical Care and Sciences, 3: 140–146.

[6] Jacoeb, A. M., Suptijah, P., & mawardi, Z. (2014). KOMPOSISI KIMIA, KOMPONEN BIOAKTIF DAN AKTIVITAS ANTIOKSIDAN BUAH LINDUR (Bruguiera gymnorrhiza). Jurnal Pengolahan Hasil Perikanan Indonesia, 16(1). https://doi.org/10.17844/jphpi.v16i1.7772

[7] Mitra, S., Naskar, N., Lahiri, S., dan Chaudhuri, P. 2023. A study on phytochemical profiling of Avicennia marina mangrove leaves collected from Indian Sundarbans. Sustainable Chemistry for the Environment, 4: 100041.

[8] Opod, A.N.T., Yamlean, P.V.Y., dan Mansauda, K.L.. 2024. Pengaruh Variasi Trietanolamin dan Asam Stearat Terhadap Stabilitas Fisik Sediaan Krim Ekstrak Etanol Daun Sirsak (Annona muricata L.). Pharmacon, 13: 393.

[9] Antara dan Megawati, F. 2022. Review Artikel: Trend Pemilihan Sediaan Kosmetik Herbal pada Kulit Wajah. Usadha, 2: 43–50.

[10] Mawardika, W. 2023. Antibacterial Potency of Jackfruit Leaf Extract (Artocarpus heterophyllus L.) Against Salmonella typhi. Jurnal Farmasi Indonesia, 20: 195–204.

[11] Himaniarwati. 2019. Optimasi Sediaan Krim Dari Ekstrak Etanol Daun Muda Pepaya (Carica papaya L.) Sebagai Antioksidan. Jurnal Mandala Pharmacon Indonesia, 5: 1–9.

[12] Setianingrum. 2025. Pengaruh Basis Krim Tipe A/M dan M/A dalam Sediaan Krim Ekstrak Etanol Batang Bajakah Tampala. Action Research Literate, 9: 1–19.

[13] Suena, N., Ariani, N., dan Antari, N. 2022. Physical Evaluation and Hedonic Test of Sandalwood Oil (Santalum album L.) Cream as an Anti-Inflammatory. Jurnal Ilmiah Medicamento, 8: 22–30.

[14] Saryanti, D., Setiawan, I., dan Safitri, R.A. 2019. Optimasi Formula Sediaan Krim M/A dari Ekstrak Kulit Pisang Kepok (Musa paradisiaca L.). Jurnal Riset Kefarmasian Indonesia, 1: 225–237.

[15] Thomas, N., Andy Suryadi, A.M., S. Latif, M., Hutuba, A.H., dan Susanti, S. 2024. Formulasi Dan Uji Stabilitas Fisik Krim Pelembab Ekstrak Rumput Laut (Eucheuma cottonii). Indonesian Journal of Pharmaceutical Education, 4: 1–9.

[16] Harborne, J. B. (1998). Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman & Hall.

[17] Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Medicinal & Aromatic Plants, 4(3), 1–6.

[18] Winarno, F. G. (2004). Kimia Pangan dan Gizi. Jakarta: Gramedia Pustaka Utama.

[19] Dai, J., & Mumper, R. J. (2010). Plant phenolics: Extraction, analysis and their antioxidant and anticancer properties. Molecules, 15(10), 7313–7352.

[20] Sasidharan, S., Chen, Y., Saravanan, D., Sundram, K. M., & Yoga Latha, L. (2011). Extraction, isolation and characterization of bioactive compounds from plants’ extracts. African Journal of Traditional, Complementary and Alternative Medicines, 8(1), 1–10.

[21] Naczk, M., & Shahidi, F. (2006). Phenolics in cereals, fruits and vegetables: Occurrence, extraction and analysis. Journal of Pharmaceutical and Biomedical Analysis, 41(5), 1523–1542.

[22] Mansur, J. S., Breder, M. N. R., Mansur, M. C. A., & Azulay, R. D. (1986). Determination of sun protection factor by spectrophotometry. Anais Brasileiros de Dermatologia, 61(3), 121–124.

[23] Saewan, N., & Jimtaisong, A. (2015). Natural products as photoprotection. Journal of Cosmetic Dermatology, 14(1), 47–63. https://doi.org/10.1111/jocd.12123

[24] Nichols, J. A., & Katiyar, S. K. (2010). Skin photoprotection by natural polyphenols: Anti-inflammatory, antioxidant and DNA repair mechanisms. Archives of Dermatological Research, 302, 71–83. https://doi.org/10.1007/s00403-009-1001-3

[25] Dutra, E. A., Oliveira, D. A. G. C., Kedor-Hackmann, E. R. M., & Santoro, M. I. R. M. (2004). Determination of sun protection factor (SPF) of sunscreens by ultraviolet spectrophotometry. Brazilian Journal of Pharmaceutical Sciences, 40(3), 381–385.

[26] Mbanga, L., Mulenga, M., Mpiana, P. T., & Bokolo, K. (2014). In vitro sun protection factor (SPF) determination of some plant extracts. International Journal of Advanced Research in Chemical Science, 1(4), 1–6.

[27] Martins, S., Mussatto, S. I., Martínez-Avila, G., Montañez-Saenz, J., Aguilar, C. N., & Teixeira, J. A. (2016). Bioactive phenolic compounds: Production and extraction by solid-state fermentation. Food Chemistry, 196, 135–142.

[28] O. V Zillich, P. Eisner, and M. Kerscher, “Polyphenols as active ingredients for cosmetic products,” pp. 455–464, 2015, doi: 10.1111/ics.12218.

[29] P. B. Sanap, P. M. Sonawane, S. A. Khairnar, and M. Raijade, “Formulation And Evaluation of Anti-oxidant Herbal Face Cream Using Pomogranate Leaves Extract,” vol. 10, no. 5, pp. 303–315, 2025.

[30] N. Kumar and N. Goel, “Phenolic acids : Natural versatile molecules with promising therapeutic applications,” Biotechnol. Reports, vol. 24, p. e00370, 2019, doi: 10.1016/j.btre.2019.e00370.

[31] M. Farhan, “The Promising Role of Polyphenols in Skin Disorders,” pp. 1–35, 2024.

[32] T. Xiao et al., “Food Chemistry : X Advances in emulsion stability : A review on mechanisms , role of emulsifiers , and applications in food,” Food Chem. X, vol. 29, no. February, p. 102792, 2025, doi: 10.1016/j.fochx.2025.102792.

[33] S. Baptista and F. Freitas, “Formulation of the Polysaccharide FucoPol into Novel Emulsified Creams with Improved Physicochemical Properties,” 2022.

[34] M. Manian, P. Jain, and D. Vora, “Formulation and Evaluation of the In Vitro Performance of Topical Dermatological Products Containing Diclofenac Sodium,” 2022.

[35] M. Ali, R. Enayatifard, M. Khalili, and M. Ghaffarloo, “Correlation between Sun Protection Factor and Antioxidant Activity , Phenol and Flavonoid Contents of some Medicinal Plants,” vol. 13, no. May 2013, pp. 1041–1047, 2014.

[36] A. Tıbbi, B. Geliştirilen, B. Güneş, and K. Güneş, “Sun Protective Potential and Physical Stability of Herbal Sunscreen Developed from Afghan Medicinal Plants,” vol. 17, no. 3, pp. 285–292, 2020, doi: 10.4274/tjps.galenos.2019.15428.

[37] A. Manigauha and B. Dubey, “Journal of Pharmaceutical Sciences & Emerging Drugs Preparation and Assessment of Sunscreen Cream Containing Extract Acquired from Plant Origin,” pp. 7–10, 2019, doi: 10.4172/2380-9477.1000132.

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Published

2026-05-04

How to Cite

Sunscreen activity of ethanol extract of mangrove fruit flour and its formulation in cream preparation. (2026). BIS Health and Environmental Science, 3, V326008. https://doi.org/10.31603/bishes.462