The effect of extraction methods on total flavonoid levels and antinflammatory activity of seagrass (enhalus acoroides) leaf extract on rabbits
Keywords:
Antiinflammatory, Infundation, Total flavonoid levels, Ultrasound assisted extraction, Enhalus acroidesAbstract
Seagrass (Enhalus acoroides) has been identified as a promising candidate for natural anti-inflammatory agents due to its content of secondary metabolites, including flavonoids, steroids, and alkaloids. The extraction method employed can significantly influence the total flavonoid content. This study aimed to quantify the total flavonoid content derived from different extraction methods and assess the anti-inflammatory activity of these extracts in rabbits, using varying concentrations of 1%, 25%, 50%, 75%, and 100%. The extraction techniques utilized in this study were infusion and Ultrasonic-Assisted Extraction (UAE). The resulting extracts underwent phytochemical screening, and total flavonoid content was quantified using the colorimetric method. The extract with the highest flavonoid concentration was subjected to an anti-inflammatory activity test on rabbits with induced burn wounds. Data were analyzed using SPSS, encompassing normality tests, homogeneity tests, and non-parametric analyses, including the Kruskal-Wallis and Mann-Whitney tests, to determine significant differences in anti-inflammatory activity across the various extract concentrations. The UAE method, using ethyl acetate as a solvent, yielded the highest flavonoid content at 68.836±10.44 mgQE/g. The anti-inflammatory activity of the extracts was evident through a reduction in burn wound size from day one to day fourteen. The Enhalus acoroides leaf extract, obtained via the UAE method with ethyl acetate and containing flavonoids, alkaloids, and steroids, exhibits significant anti-inflammatory activity in rabbits (Oryctolagus cuniculus). There was no statistically significant difference (p > 0.05) in the reduction of wound diameter between the 50%, 75%, and 100% extract concentrations and the positive control (Bioplacenton®) over the fourteen-day treatment period.
References
[1] Sari Dina Permata, Sari Gigih Kenanga, and Saraswati Maulita, “Uji Aktivitas Gel Ekstrak Daun Kelor (Maringa Oelifera L.) dari Kabupaten Blora Terhadap Penyembuhan Luka Bakar PD Kelinci,” Joseph (Journal of Science and Pharmacy), vol. 2, pp. 28–41, 2022.
[2] W. R. B. Santosa and R. Anggraini, “Perbandingan Efektifitas Tumbukan Daun Cocor Bebek dan Rebusan Daun Sirih Terhadap Penyembuhan Luka Bakar Derajat II Pada Tikus Wistar Jantan,” Jurnal Insan Cendekia, vol. 8, no. 1, pp. 74–79, 2021.
[3] G. R. W. Sanjaya, N. M. Linawati, I. G. K. N. Arijana, I. A. I. Wahyuniari, and I. G. N. S. Wiryawan, “Flavonoid dalam Penyembuhan Luka Bakar pada Kulit: Flavonoids in Healing Burns on the Skin,” Jurnal Sains dan Kesehatan, vol. 5, no. 2, pp. 243–249, 2023.
[4] S. Hidayati, F. Oktavianti, D. A. Susanti, and Q. Aini, “Aktivitas Antiinflamasi In Vitro dan In Vivo Ekstrak Etanol Daun Mangga Arumanis (Mangifera indica L.): In Vitro and In Vivo Anti-Inflamatory Activities of Ethanol Extract Mangifera indica L. Leaves,” Jurnal Sains dan Kesehatan, vol. 4, no. 5, pp. 488–494, 2022.
[5] E. R. Taminggu and T. Tahril, “Identifikasi Senyawa Metabolit Sekunder Pada Batang dan Daun Lamun (Seagrass) di Teluk Palu,” Media Eksakta, vol. 18, no. 1, pp. 6–11, 2022, doi: 10.22487/me.v18i1.1016.
[6] M. Yusuf, I. Sari, and A. Wijaya, “Efek Antiinflamasi Ekstrak Etanol Daun Lamun (Enhalus acoroides) Terhadap Mencit (Mus musculus) Jantan Yang Diinduksi Karagen,” Jurnal Ilmiah Manuntung, vol. 7, no. 2, pp. 165–174, 2021.
[7] I. D. Rahakbauw and T. Watuguly, “Analisis Senyawa Flavonoid Daun Lamun Enhalus Acoroides di Perairan Pantai Desa Waai Kabupaten Maluku Tengah,” BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan, vol. 3, no. 1, pp. 53–62, 2016, doi: 10.30598/biopendixvol3issue1page53-62.
[8] F. Maryam, Y. P. Utami, S. Mus, and R. Rohana, “Perbandingan Beberapa Metode Ekstraksi Ekstrak Etanol Daun Sawo Duren (Chrysophyllum cainito L.) Terhadap Kadar Flavanoid Total Menggunakan Metode Spektrofotometri UV-VIS,” Jurnal Mandala Pharmacon Indonesia, vol. 9, no. 1, pp. 132–138, 2023, doi: 10.35311/jmpi.v9i1.336.
[9] E. Yolandari, S., & Mustiqawati, “Uji Efek Ekstrak Etanol Daun Jambu Biji (Psidium Guajava L) Sebagai Antiinflamasi Pada Kelinci (Oryctolagus Cuniculus),” JURNAL Promotif Preventif, vol. 5, no. 1, pp. 117–129, 2022.
[10] S. R. Lamadjido, U. Umrah, and J. Jamaluddin, “Formulasi dan Analisis Nilai Gizi Bakso Kotak dari Jamur Tiram Putih (Pleurotus Ostreatus),” Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), vol. 5, no. 2, pp. 166–174, 2019, doi: 10.22487/j24428744.2019.v5.i2.13149.
[11] N. Pawarti, M. Iqbal, D. A. Ramdini, and C. Yuliyanda, “Pengaruh Metode Ekstraksi Terhadap Persen Rendemen dan Kadar Fenolik Ekstrak Tanaman yang Berpotensi sebagai Antioksidan The Effect of Extraction Methods on Percent Yield and Phenolic Content of Plant Extracts Potentially as Antioxidants,” Jurnal Medula, vol. 13, no. 4, pp. 590–593, 2023.
[12] E. Pujiastuti and D. El’Zeba, “Perbandingan Kadar Flavonoid Total Ekstrak Etanol 70% Dan 96% Kulit Buah Naga Merah Hylocereus Polyrhizus) dengan Spektrofotometri,” Cendekia Journal of Pharmacy, vol. 5, no. 1, pp. 28–43, 2021, doi: 10.31596/cjp.v5i1.131.
[13] R. R. Kamilah, K. S. Rahmasari, W. Wirasti, and V. A. Nur, “The Effect of Solvent Extraction in Making Natural Sweetener from Lemba Fruit ( Curculigo latifolia ) on Total Sugar Content,” The 16th University Research Colloqium, pp. 714–722, 2022.
[14] Nurafni and R. M. Nur, “Identifikasi Senyawa Bioaktif Jenis-Jenis Lamun Di Perairan Pulau Morotai,” Seminar Nasional Pendidikan Biologi Kepulauan Aula Banau, vol. 18, no. 20, pp. 26–32, 2018.
[15] Hasan, Suryadi, and Djufri, “Uji Aktivitas Antidiabetes Ekstrak Etil Asetat Daun Lamun ( Enhalus acoroides ) Pada Mencit ( Mus musculus ),” Journal Syifa Sciences and Clinical Research (JSSCR), vol. 4, no. 1, pp. 293–305, 2022.
[16] N. R. K. Nisaa and V. H. , Abd. Malik, “Analisis Kadar Total Flavonoid Ekstrak Etanol Kulit Pisang Cavendish (Musa paradisiaca var. Sapientum) Menggunakan Metode Spektrofotometri Uv-Vis,” Jurnal Sains dan Kesehatan, vol. 3, no. 4, pp. 586–592, 2021.
[17] N. K. L. Puspa Yani, K. Nastiti, and N. Noval, “Pengaruh Perbedaan Jenis Pelarut Terhadap Kadar Flavonoid Total Ekstrak Daun Sirsak (Annona muricata L.),” Jurnal Surya Medika, vol. 9, no. 1, pp. 34–44, 2023, doi: 10.33084/jsm.v9i1.5131.
[18] E. F. Yanti and N. Purwanti, “Penetapan Kadar Falvonoid Total Dan Uji Aktivitas Ekstrak Etanol Daun Makadamia (Macadamia integrifolia) Dengan Metode DPPH,” Journal of Islamic Pharmacy, vol. 7, no. 2, pp. 100–103, 2023, doi: 10.18860/jip.v7i2.17522.
[19] A. Chahyadi and Elfahmi, “The influence of extraction methods on rutin yield of cassava leaves (Manihot esculenta Crantz),” Saudi Pharmaceutical Journal, vol. 28, no. 11, pp. 1466–1473, 2020, doi: 10.1016/j.jsps.2020.09.012.
[20] D. Y. Wiratma, K. Manurung, S. Supartiningsih, and M. F. Telaumbanua, “Uji Aktivitas Ekstrak Etanol Bawang Putih (Alliumsativum L.)Yang Dihitamkan Sebagai Anti Inflamasi yang Diinduksi Oleh Karagenan Terhadap Mus musculus,” Jurnal Farmanesia, vol. 4, no. 2, pp. 116–121, 2017, doi: 10.51544/jf.v4i2.2712.
[21] M. F. Almaidah, “Uji Aktivitas Anti Inflamasi Ekstrak Etanol Daun Buni (Antidesma Bunius L. Spreng) Terhadap Tikus Putih (Rattus Norvegicus),” Pakistan Research Journal of Management Sciences, vol. 7, no. 5, pp. 1–2, 2018.
[22] T. Carolina, D. Fitri Nugraha, and U. H. Fetriyah, “Activity Test Of Chloroform Extract Sangkareho (Callicarpa Longifolia Lam.) Leaf On Wound Healing In Male Wistar Rats,” Jurnal Surya Medika, vol. 7, no. 2, pp. 166–173, 2022.
[23] S. Lallo, B. Hardianti, H. Umar, W. Trisurani, A. Wahyuni, and M. Latifah, “Aktivitas Anti Inflamasi dan Penyembuhan Luka dari Ekstrak Kulit Batang Murbei (Morus alba L.),” Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), vol. 6, no. 1, pp. 26–36, 2020, doi: 10.22487/j24428744.2020.v6.i1.14661.
[24] N. Sari, M. Latief, P. S. Farmasi, P. S. Kimia, and U. Jambi, “Uji Aktivitas Ekstrak Etanol Daun Sungkai (Peronema Canescens Jack) Terhadap Penyembuhan Luka Bakar Pada Kelinci Jantan (Oryctolagus cuniculus),” Indonesian Journal of Pharma Science, vol. 4, no. 1, p. 11, 2022.
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